Determination of endotheliocites destruction as a marker of vascular damage in ageing males

##plugins.themes.bootstrap3.article.sidebar##

PDF (Українська)
DOI: https://doi.org/10.30841/2307-5090.2(57).2016.82951
Keywords:
androgen deficiency in ageing males, markers of endothelial dysfunction, destruction of endothelial cells,

##plugins.themes.bootstrap3.article.main##

Г. Л. Пустовойт
ВДНЗ «Українська медична стоматологічна академія», м. Полтава, Ukraine

Abstract

The objective: is to evaluate the diagnostic value of the destruction of endothelial cells by calculating the levels of circulating CD32+, CD40+-microparticles as a marker of endothelial dysfunction in ageing males. Patients and methods. the study involved 64 men aged 45 to 76 years. The study group consisted of 40 men suffering from type 2 diabetes mellitus (DM-2) in combination with arterial hypertension (AH) and the control group – 24 patients without the above-mentioned diseases. In both groups, the patients were divided into two subgroups: the subgroup of I – a persons of middle age (45–59 years), II subgroup – persons  60 years. The level of circulating endothelial cells was determined by counting CD32+, CD40+-microparticles (endothelial specific markers) by flow cytometry, endothelin levels in serum were determined by ELISA. Results. Both in the main and control groups elderly patients showed an increase in the number of circulating CD32+, CD40+microparticles compared with those of middle age: 5,06±1,92•107/L vs. 4,76±1,87•107/L (p>0,05) and 2,2±0,2•107/L vs. 1,99±0,3•107/L, respectively (p>0,05). Regardless of age in the study group the number of circulating CD32+, CD40+-microparticles were more than 2 times higher than in the control group: in the I subgroup 4,76±1,87•107/L vs. 1,99±0,3•107/L (p<0,01) in the subgroup II –5,06±1,92•107/L vs. 2,2±0,2•107/L, respectively (p<0,05), indicating the correlation of type 2 diabetes mellitus (DM-2) and hypertension with damage of the endothelium. Regardless to age in men suffering from type 2 diabetes and hypertension, a higher level of endotelin compared with control group was revealed: 3,92±0,7 fmol/mL vs 1,82±0,27 fmol/mL (p<0,05) and 2,06±0,6 fmol/mL vs 1,28±0,25 fmol/mL respectively (p<0,05). Conclusion. Increased levels of circulating CD32+, CD40+microparticles simultaneously with increased levels of endothelin in ageing males with type 2 diabetes and hypertension show that these markers can be evaluated as indicators of endothelial destruction and dysfunction.

##plugins.themes.bootstrap3.article.details##

How to Cite
Пустовойт, Г. Л. (2016). Determination of endotheliocites destruction as a marker of vascular damage in ageing males. Health of Man, (2(57), 60–62. https://doi.org/10.30841/2307-5090.2(57).2016.82951
Issue
No. 2(57) (2016)
Section
For practicing physicians
Copyright (c) 2020 Г. Л. Пустовойт

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors retain the copyright and grant the journal the first publication of original scientific articles under the Creative Commons Attribution 4.0 International License, which allows others to distribute work with acknowledgment of authorship and first publication in this journal.

Author Biography

Г. Л. Пустовойт, ВДНЗ «Українська медична стоматологічна академія», м. Полтава

А.Л. Пустовойт

References

Бувальцев В.И. Дисфункция эндотелия как новая концепция профилактики и лечения сердечно-сосудистых заболеваний / В.И. Бувальцев // Международный мед. журнал. – 2001. – № 3. – С. 9–14.

Верткин А.Л. Дефицит тестостерона и соматическая патология / А.Л. Верткин, Л.Ю. Моргунов, Е.Н. Аринина [и др.] // Лечащий врач. – 2006. – № 10. – С. 34–38.

Войтенко В.П. Україна в європейському контексті: смертність від головних причин / В.П. Войтенко, А.В. Писарук, Н.М. Кошель, М.Г. Ахаладзе // Пробл. старения и долголетия. – 2012. – Т. 21, № 2. – С. 191–210.

Дедов И.И. Возрастной андрогенный дефицит у мужчин / И.И. Дедов, С.Ю. Калиниченко. – М.: Практическая медицина, 2006. – 240 с.

Захаров Д.И. Норма, болезнь и вопросы здравоохранения / Д.И. Захаров. – М.: Знание, 2000. – 144 с.

Куценко Н.Л. Определение содержания циркулирующих CD32+, CD40+-микрочастиц – модификация метода оценки деструкции эндотелиальных клеток / Н.Л. Куценко, Л.Г. Савченко, Э.И. Кайдашева и др. // Клиническая лабораторная диагностика. – 2011. – № 7. – С. 20–23.

Коркушко О.В. Эндотелиальная дисфункция. Клинические аспекты проблемы / О.В. Коркушко, В.Ю. Лишневская // Кровообіг та гемостаз. – 2003. – № 2. – С. 4–15.

Коробейніков Г.В. Біологічні механізми старіння і рухова активність людини / Г.В. Коробейніков // Фізична активність, здоров’я і спорт. – 2010. – № 2. – С. 3–13.

Печерский А.В. Влияние частичного возрастного андрогенного дефицита (PADAM) на импульсный режим инкреции некоторых гормонов и митотическую активность / А.В. Печерский, В.Ф. Семиглазов, О.Б. Лоран [и др.] // Цитология. – 2006. – № 48 (10). – С. 862–866.

Boos C.J. Circulating endothelial cells, arterial stiffness, and cardiovascular risk stratification in Hipertension / C.J. Boos, D.A. Lane, M. Karpha et al. // Chest. – 2007. – Vol. 132. – P. 1540–1547.

Eiselein L. Lipolysis products from triglyceride – rich lipoproteins increase endothelial permeability, perturb zonula occludens-1 and F-actin, and inuce apoptosis / L. Eiselein, D.W. Wilson, M.W. Lame, J.C. Rutledge // Am. J. Physiol. – Heart Circ. Physiol. – 2007. – Vol. 292. – P. 2745–2753.

Ferrini R.J.L. Sex hormones and age: a crosssectional study of testosterone and estradiol and their bioavailable fractions in community-dwelling men / R.J.L. Ferrini, E. Barrett-Connor // Am. J. Epidemiol. – 1998. – Vol. 147 (8). – P. 750–754.

Jean-Baptiste M. NO (Nitric oxide) and cardiovascular Homeostasis / M. JeanBaptiste // Menarini International Industrie Farmaceutiche Riunite s.r.l., Paris. – 1999. – Р. 221–226.

Langer С. Testosterone up-regulates scavenger receptor BI and stimulates cholesterol efflux from macrophages / С. Langer, B. Gansz, С. Goepfert et al. // Biochem. Biophys. Res Commun. – 2002. – Vol. 296 (5). – P. 1051–1057.

Rossi M.С.Е. Identifying patients with type 2 diabetes at high risk of microalbuminuria: results of the DEMAND (Developing Education on Microalbuminuria for Awareness of renal and cardiovascular risk in Diabetes) Study / M.С.Е. Rossi, A. Nicolucci, F. Pellegrini [et al.] // Nephrol. Dial. Transplant. – 2008. – Vol. 23, № 4. – P. 1278–1284.

Weinberg J.M. Lipotoxicity / J.M. Weinberg // Kidney Int. – 2006. – № 70. – P. 1560–1566.