Possibilities of correction of protein peroxidation and antioxidant defense in patients with urolithiasis
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Abstract
The objective: research the condition of prooxidant system and the system of antioxidant defense in patients with urolothiasis and antioxidant activity of phytomedications, in particular in calcium-oxalate nephrolithiasis.
Patients and methods. Materials and methods. 56 patients, which were examined and treated we divided into groups: 1 group (n=36) patients with urolithiasis aged from 18 to 65 years; 2 group (n=20), which is a control group. 20 healthy people of the same age are in this group. In first group, in complex treatment of patients with urolithiasis was included phytomedication. Medication was prescribed by по 45 ml 3 times per day for 1 month. For study the condition of peroxidation of proteins we researched indexes of oxidative modification of proteins. For study of the state of peroxidation of proteins we researched the indexes of oxidative modification of proteins according to the method of Dubinina Dubinina E.E. and coauthors. Activity of superoxide dismutase we identified according to the method of Chevari Chevari S. and coauthors. Assay of catalase in blood serum was performed by the method of Bah Bah A.N. and Zubkov Zubkov S.
Results. Found that in patients with urolithiasis occurs oxidative activation, shown a significant increase in the level of oxidation-modified proteins. However, in all patients antioxidant defense weakened, which demonstrates a significant reduction in enzyme activity of superoxide dismutase and especially catalase. After treatment we indicated the tendency to significantly decrease of oxidation-modified proteins in our patients compared to the original level, which shows the antioxidant features of phytomedication. Also usage of phytomedication leads to improvement of functional capacity of antioxidant system.
Conclusion. In patients with calcium-oxalate nephrolithiasis there is development of oxidative stress, which appears a significant increase in content and accumulation of protein peroxidation products amid growing tension adjustment mechanisms of antioxidant defense system. Using of complex therapy with inclusion of proposed phytomedication allows adjust methabolic disorders, which manifested by recovery of antioxidative activity of blood serum, decrease of the oxidative modification of proteins system.
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References
Возианов А. Ф. Атлас: руководство по урологии: в 3 т. / А. .Ф. Возианов, А. В. Люлько. – Днепропетровск: РИА «Днепр-VAL», 2001. – 195 с.
Литвинець Є.А., Зеляк М.В., Соломчак Д.Б. та ін. Стан перекисного окислення ліпідів і антиоксидантного захисту при патології сечовидільної системи та доцільність використання біофлавоноїдів у комплексному лікуванні // Галицький лікарський вісник. – 2004 . – No 3. – С. 110–113.
Мочекаменная болезнь: этиопатогенез, диагностика, лечение и метафилактика: пособие / В. И. Вощула [и др.]; под. общ. ред. В. И. Вощулы. – Минск: Зималето, 2010. – 220 с.
Карімов Карімов І.З. Окисна модифікація білків і перекисне окислення ліпідів у розвитку метаболічної інтоксикації при патології / І.З. Карімов // Лабораторна діагностика. – 2005. – No 1 (31). – С. 7–13.
Соодаева С.К. Окислительный стресс и антиоксидантная терапия при заболеваниях органов дыхания / С.К. Соодаева // Пульмонология. – 2006. – No 5. – С. 122–126.
Черненко В.В. Підвищення ефективності реабілітації у хворих на сечокам’яну хворобу після проведення літотрипсії /В.В. Черненко, Д.В. Черненко //Урологія. – 2015. – No 4. – С. 14–20.
Borysewicz-Sanczyk H. Urolithiasis risk factors in obese and overweight children / H. Borysewicz-Sanczyk, T. Porowski, A. Hryniewicz // Pediatr Endocrinol Diabetes Metab. – 2012. – Vol. 18, N 2. – P. 53–57.
Knoll T. Epidemiology, Pathogenesis, and Pathophysiology of Urolithiasis / T. Knoll // Eur. Urol. Suppl. – 2010. – Vol. 9. – P. 802–806.
Elsas L.J. Approach to inborn emors of metabolism / L.J. Elsas, L. Gladman, D. Ausiello // Cecil Medicine. Philadelphia, Pa: Saunders Elsevier. – 2008. – P. 216.
Rink L. Zinc-altered immune function and cytokine production / L. Rink, H. Kirchner // J. Nutr. 2008. – Vol. 130. – P. 1407–1411.
Pearle M.S. Urologic diseases in America project: Urolithiasis / M.S. Pearle, E.A. Calhoun, G.C. Curhan // J. Urol. – 2005. – Vol. 173. – P. 848–857.
Williams K.J., Fisher E.A. Oxidation, lipoproteins and atherosclerosis // Curr. Opin. Clin. Nutr. Care. – 2005. – Vol. 8. – P. 139–147.
