Peculiarities of Hystochemical Glutamatedehydrogenase Changes in Artificial Urinary Bladder in Dynamics (Experimental Study)

Р. В. Савчук, Ф. І. Костєв, Б. А. Насибуллін


The gold standard for the treatment of muscle-invasive bladder cancer with the best indicators of the patient’s quality of life in the postoperative period is radical cystectomy with ileocystoplasty.

The objective: is the investigation of the hystochemically revealed activity of glutamate dehydrogenase in the wall of artificial bladder in experimental animals.

Materials and methods. Experimental studies were performed on 18 female mini-pigs aged 4–5 months and a mass of 8–10 kg. Modeling of the artificial bladder was performed by cystectomy followed by ileocystoplasty. Control studies of glutamate dehydrogenase were performed at 3, 6 and 12 months after the operational simulation.

Results. Hystoenzymological studies of the ileum wall at different stages of the study after the experimental modeling of neobladder did not reveal statistically significant changes in the activity of GDH in the epithelial and submucosa layers compared to healthy animals.

Conclusion. The activity of GDG in the muscular layer of the neocyst after 3 months was significantly increased, with a subsequent tendency to increase. The hypertrophy of myocytes changes the transport capabilities of the neocyst system and leads to a shift in the emphasis of substrata provision towards intermediate metabolites. The insufficient general support contributes to the preservation of life-support processes at a relatively low level.


radical cystectomy; ileocystoplasty; glutamate dehydrogenase


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